Application
Western blot analysis of extracts from NIH-3T3 tissue using MAP3K7 (Phospho-Thr187) Antibody. The lane on the right is treated with the antigen-specific peptide.| Product Name | MAP3K7 (phospho Thr187) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | M3K7, MAP3K7, Mitogen-activated protein kinase kinase kinase 7, TGF-beta- activated kinase 1, kinase TAK1 |
| Product description | Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. Plays an important role in the cascades of cellular responses evoked by changes in the environment. Mediates signal transduction of TRAF6, various cytokines including interleukin-1 (IL-1), transforming growth factor-beta (TGFB), TGFB-related factors like BMP2 and BMP4, toll-like receptors (TLR), tumor necrosis factor receptor CD40 and B-cell receptor (BCR). Ceramides are also able to activate MAP3K7/TAK1. Once activated, acts as an upstream activator of the MKK/JNK signal transduction cascade and the p38 MAPK signal transduction cascade through the phosphorylation and activation of several MAP kinase kinases like MAP2K1/MEK1, MAP2K3/MKK3, MAP2K6/MKK6 and MAP2K7/MKK7.1) Shin MS, et al. (2009)Biochim Biophys Acta 1793, 1156-64 |
| Immunogen | MAP3K7 (phospho Thr187) antibody was raised against a peptide sequence around phosphorylation site of threonine 187 (H-M-T (p)-N-N) derived from Human MAP3K7. |
| Modification | p-Thr187 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| Western Blot: 1:500~1:1000: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MAP3K7 |
|---|---|
| Alternative Names | M3K7 MAP3K7 Mitogen-activated protein kinase kinase kinase 7 TGF-beta- activated kinase 1 kinase TAK1 |
| Molecular Weight(MW) | 60 kDa |
| Tissue Specificity | The antibody detects endogenous level of IMAP3K7 only when phosphorylated at threonine 187. |
| Entrez Gene | 6885 |
|---|---|
| Protein Accession | NP_003179.1 |
Application
Western blot analysis of extracts from NIH-3T3 tissue using MAP3K7 (Phospho-Thr187) Antibody. The lane on the right is treated with the antigen-specific peptide.| Application Notes | Western Blot: 1:500~1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
