Application
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with P38 delta antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Antibody is used in Western blot to detect P38 delta in nocodazole-treated HCT116 (lane 1) and PMA-treated Pam212 (lane 2) cell lysates.| Product Name | MAPK13 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | P38 delta is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is closely related to p38 MAP kinase, both of which can be activated by proinflammatory cytokines and cellular stress. MAP kinase kinases 3, and 6 can phosphorylate and activate this kinase. Transcription factor ATF2, and microtubule dynamics regulator stathmin have been shown to be the substrates of this kinase.1) Duyndam, M.C., et al., J. Biol. Chem. 278(9):6885-6895 (2003). Efimova, T., et al., J. Biol. Chem. 277(35):31753-31760 (2002). Knebel, A., et al., EMBO J. 20(16):4360-4369 (2001). Hu, M.C., et al., J. Biol. Chem. 274(11):7095-7102 (1999). Parker, C.G., et al., Biochem. Biophys. Res. Commun. 249(3):791-796 (1998). |
| Immunogen | This MAPK13 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 335-365 amino acids from the C-terminal region of human MAPK13. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-P,WB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 | |
| Species Reactivity | Human,Monkey,Mouse,Rat |
| Concentration | 1mg/ml |
| Purification | Unpurified |
| Gene Symbol | MAPK13 |
|---|---|
| Alternative Names | Mitogen-activated protein kinase 13 MAP kinase 13 MAPK 13 Mitogen-activated protein kinase p38 delta MAP kinase p38 delta Stress-activated protein kinase 4 MAPK13 PRKM13 SAPK4 |
| Molecular Weight(MW) | 42 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine |
Application
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with P38 delta antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Antibody is used in Western blot to detect P38 delta in nocodazole-treated HCT116 (lane 1) and PMA-treated Pam212 (lane 2) cell lysates.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
