Application
Western Blot at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: HepG2 whole cell lysate Lane 3: PC-12 whole cell lysate Lysates/proteins at 20 ug per lane.Application
Western blot analysis in Jurkat cell line and mouse kidney tissue lysates (35ug/lane).Application
Western Blot at 1:2000 dilution Lane 1: HepG2 whole cell lysate Lane 2: Hela whole cell lysate Lane 3: PC-12 whole cell lysate Lane 4: Jurkat whole cell lysate Lane 5: MCF-7 whole cell lysate Lane 6: mouse kidney lysate Lysates/proteins at 20 ug per lane.Application
Overlay histogram showing Hela cells stained with Antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit Ig| Product Name | MAPK14 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | MAPK14 is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various environmental stresses and proinflammatory cytokines. The activation requires its phosphorylation by MAP kinase kinases (MKKs), or its autophosphorylation triggered by the interaction of MAP3K7IP1/TAB1 protein with this kinase. The substrates of this kinase include transcription regulator ATF2, MEF2C, and MAX, cell cycle regulator CDC25B, and tumor suppressor p53, which suggest the roles of this kinase in stress related transcription and cell cycle regulation, as well as in genotoxic stress response.1) Cheung, P.C., et al., EMBO J. 22(21):5793-5805 (2003). Dean, J.L., et al., J. Biol. Chem. 278(41):39470-39476 (2003). Sun, A., et al., Exp. Neurol. 183(2):394-405 (2003). Yustein, J.T., et al., Oncogene 22(40):6129-6141 (2003). Frevel, M.A., et al., Mol. Cell. Biol. 23(2):425-436 (2003). |
| Immunogen | This MAPK14 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 301-330 amino acids from human MAPK14. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACS,WB |
| For FACS starting dilution is: 1:25 For WB starting dilution is: 1:2000 | |
| Species Reactivity | Human,Mouse,Rat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MAPK14 |
|---|---|
| Alternative Names | Mitogen-activated protein kinase 14 MAP kinase 14 MAPK 14 Cytokine suppressive anti-inflammatory drug-binding protein CSAID-binding protein CSBP MAP kinase MXI2 MAX-interacting protein 2 Mitogen-activated protein kinase p38 alpha MAP kinase p38 al |
| Molecular Weight(MW) | 41 kDa |
Application
Western Blot at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: HepG2 whole cell lysate Lane 3: PC-12 whole cell lysate Lysates/proteins at 20 ug per lane.
Application
Western blot analysis in Jurkat cell line and mouse kidney tissue lysates (35ug/lane).
Application
Western Blot at 1:2000 dilution Lane 1: HepG2 whole cell lysate Lane 2: Hela whole cell lysate Lane 3: PC-12 whole cell lysate Lane 4: Jurkat whole cell lysate Lane 5: MCF-7 whole cell lysate Lane 6: mouse kidney lysate Lysates/proteins at 20 ug per lane.
Application
Overlay histogram showing Hela cells stained with Antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit Ig| Application Notes | For FACS starting dilution is: 1:25 For WB starting dilution is: 1:2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
Data sheet for OM284158