Application
Formalin-fixed and paraffin-embedded human kideny carcinoma tissue reacted with ERK5 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma; HC = hepatocarcinoma.Application
Western blot analysis of anti-ERK5 C-term Pab in K562 cell line lysates (35ug/lane)| Product Name | MAPK7 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Mitogen-activated protein kinase 7, MAP kinase 7, MAPK 7, Big MAP kinase 1, BMK-1, Extracellular signal-regulated kinase 5, ERK-5, MAPK7, BMK1, ERK5, PRKM7 |
| Product description | MEK5 and ERK5, a member of the MAP kinase subfamily of the Ser/Thr protein kinases, interact specifically with one another and not with MEK1/ERK1 or MEK2/ERK2 pathways. ERK5 is activated by tyrosine and threonine phosphorylation It is expressed in many adult tissues, abundantly in heart, placenta, lung, kidney and skeletal muscle, but is not detectable in liver. The second proline-rich region may interact with actin targeting the kinase to a specific location in the cell. ERK5 is autophosphorylated on threonine and tyrosine residues when the C-terminal part of the kinase, which could have a regulatory role, is absent.1) Zhou, G., et al., J. Biol. Chem. 270(21):12665-12669 (1995). |
| Immunogen | This MAPK7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 776-805 amino acids from the C-terminal region of human MAPK7. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | MAPK7 |
|---|---|
| Alternative Names | Mitogen-activated protein kinase 7 MAP kinase 7 MAPK 7 Big MAP kinase 1 BMK-1 Extracellular signal-regulated kinase 5 ERK-5 MAPK7 BMK1 ERK5 PRKM7 |
| Molecular Weight(MW) | 88 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine |
Application
Formalin-fixed and paraffin-embedded human kideny carcinoma tissue reacted with ERK5 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma; HC = hepatocarcinoma.
Application
Western blot analysis of anti-ERK5 C-term Pab in K562 cell line lysates (35ug/lane)| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
