Application
Western blot analysis of lysate from Hela cell line, using JNK1 antibody at 1:1000 at each lane.Application
JNK1-T183/Y185 antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the LJNK1-T183/Y185 antibody for immunohistochemistry.| Product Name | MAPK8 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Mitogen-activated protein kinase 8, MAP kinase 8, MAPK 8, JNK-46, Stress-activated protein kinase 1c, SAPK1c, Stress-activated protein kinase JNK1, c-Jun N-terminal kinase 1, MAPK8, JNK1, PRKM8, SAPK1, SAPK1C |
| Product description | The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various cell stimuli, and targets specific transcription factors, and thus mediates immediate-early gene expression in response to cell stimuli. The activation of this kinase by tumor-necrosis factor alpha (TNF-alpha) is found to be required for TNF-alpha induced apoptosis. This kinase is also involved in UV radiation induced apoptosis, which is thought to be related to cytochrom c-mediated cell death pathway. Studies of the mouse counterpart of this gene suggested that this kinase play a key role in T cell proliferation, apoptosis and differentiation. Four alternatively spliced transcript variants encoding distinct isoforms have been reported.1) Dasgupta, J., et al. J. Cell. Physiol. 225(1):52-62(2010) |
| Immunogen | This MAPK8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 159-195 amino acids from human MAPK8. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MAPK8 |
|---|---|
| Alternative Names | Mitogen-activated protein kinase 8 MAP kinase 8 MAPK 8 JNK-46 Stress-activated protein kinase 1c SAPK1c Stress-activated protein kinase JNK1 c-Jun N-terminal kinase 1 MAPK8 JNK1 PRKM8 SAPK1 SAPK1C |
| Molecular Weight(MW) | 48 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Mouse |
Application
Western blot analysis of lysate from Hela cell line, using JNK1 antibody at 1:1000 at each lane.
Application
JNK1-T183/Y185 antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the LJNK1-T183/Y185 antibody for immunohistochemistry.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
