Application
Western blot analysis of MAPKAP1 in human skeletal muscle tissue lysate with MAPKAP1 antibody at (A) 1 and (B) 2 μg/mL.Application
Immunohistochemistry of MAPKAP1 in human skeletal muscle tissue with MAPKAP1 antibody at 5 μg/mL.| Product Name | MAPKAP1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | MAPKAP1 Antibody: MAPKAP1 was initially identified as the human homolog of S. pombe SIN1. Recent evidence has shown that it identical to Mip1, a protein that interacts with MEKK2, a member of the mitogen-activated protein kinase (MAPK) intracellular signaling network. MAPKAP1 is thought to prevent MEKK2 activation and dimerization by forming a complex with the inactive and non-phosphorylated MEKK2, thereby blocking the JNK1/2, ERK1/2, p38 and ERK5 MAPKs. MAPKAP1 has also been shown to play a role in the TOR signaling process, a pathway that is involved in controlling cell growth and proliferation in response to environmental cues such as nutrients, growth factors and hormones. Experiments showed that MAPKAP1 helped to maintain the TOR/rictor assembly but not the TOR/RAPTOR complex, which allowed specific phosphorylation of Akt, a kinase that is believed to couple the growth factor-PI3K signaling pathway to the nutrient-regulated TOR signaling pathway. Multiple alternatively spliced isoforms of MAPKAP1 have been identified.1) Schroder W, Cloonan N, Bushell G, et al. Alternative polyadenylation and splicing of mRNAs transcribed from the human Sin1 gene. Gene2004; 339:17-23. |
| Immunogen | MAPKAP1 antibody was raised against a 18 amino acid synthetic peptide from near the center of human MAPKAP1. The immunogen is located within amino acids 340 - 390 of MAPKAP1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIHC-PWB |
| MAPKAP1 antibody can be used for detection of MAPKAP1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MAPKAP1 |
|---|---|
| Alternative Names | MAPKAP1 Antibody: MIP1 SIN1 JC310 SIN1b SIN1g MIP1 Target of rapamycin complex 2 subunit MAPKAP1 Mitogen-activated protein kinase 2-associated protein 1 TORC2 subunit MAPKAP1 |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Sheep: (100%) |
| Entrez Gene | 79109 |
|---|---|
| Protein Accession | NP_001006618 |
Application
Western blot analysis of MAPKAP1 in human skeletal muscle tissue lysate with MAPKAP1 antibody at (A) 1 and (B) 2 μg/mL.
Application
Immunohistochemistry of MAPKAP1 in human skeletal muscle tissue with MAPKAP1 antibody at 5 μg/mL.| Positive Control | 1) Cat. No. 1375 - Human Skeletal Muscle Tissue Lysate |
|---|---|
| Application Notes | MAPKAP1 antibody can be used for detection of MAPKAP1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | MAPKAP1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | MAPKAP1 Antibody is supplied in PBS containing 0.02% sodium azide. |
