Application
Immunohistochemical analysis of paraffin-embedded human brain tissue using MAPKAPK2 (Phospho-Ser272) antibody (left) or the same antibody preincubated with blocking peptide (right).Application
Western blot analysis of extracts from RAW264.7 cells treated with UV using MAPKAPK2 (Phospho-Ser272) Antibody. The lane on the right is treated with the antigen-specific peptide.| Product Name | MAPKAPK2 (phospho Ser272) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | This gene encodes a member of the Ser/Thr protein kinase family. This kinase is regulated through direct phosphorylation by p38 MAP kinase. In conjunction with p38 MAP kinase, this kinase is known to be involved in many cellular processes including stress and inflammatory responses, nuclear export, gene expression regulation and cell proliferation. Heat shock protein HSP27 was shown to be one of the substrates of this kinase in vivo. Two transcript variants encoding two different isoforms have been found for this gene.1) Zu Y.-L., Biochem. Biophys. Res. Commun. 200-1118-1124(1994). |
| Immunogen | MAPKAPK2 (phospho Ser272) antibody was raised against a peptide sequence around phosphorylation site of Serine 272 (A-I-S (p)-P-G) derived from Human MAPKAPK2. |
| Modification | p-Ser272 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100 | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MAPKAPK2 |
|---|---|
| Alternative Names | MAPK2 MAPKAPK2 RPS6KC1 |
| Molecular Weight(MW) | 45 kDa |
| Tissue Specificity | The antibody detects endogenous levels of MAPKAPK2 only when phosphorylated at serine 272. |
| Entrez Gene | 9261 |
|---|---|
| Protein Accession | NP_116584.2 |
Application
Immunohistochemical analysis of paraffin-embedded human brain tissue using MAPKAPK2 (Phospho-Ser272) antibody (left) or the same antibody preincubated with blocking peptide (right).
Application
Western blot analysis of extracts from RAW264.7 cells treated with UV using MAPKAPK2 (Phospho-Ser272) Antibody. The lane on the right is treated with the antigen-specific peptide.| Application Notes | Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
