Application
Antibody used in WB on N2A at 1:2000 (Lane 1: 10ug N2A expressed human Tau-C Lane 2: 10ug N2A expressed human Tau-E Lane 3: 10ug N2A expressed human Tau-F Lane 4: 10ug N2A expressed human Tau-B Lane 5: 10ug N2A expressed human Fetal-Tau Lane 6: 10ug N2A expressed human Tau-D Lane 7: 10ug N2A expressed human much Fetal-Tau Lane 8: 10ug N2A lysate ).Application
Antibody used in WB on Human HepG2 at 0.2-1 ug/ml.Application
Antibody used in IHC on mouse cortex at: 1:1000.| Product Name | MAPT Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | MAPT is differentially expressed in the nervous system, depending on stage of neuronal maturation and neuron type. The mutations in the gene have been associated with several neurodegenerative disorders such as Alzheimer's disease, Pick's disease, frontotemporal dementia, cortico-basal degeneration and progressive supranuclear palsy.This gene encodes the microtubule-associated protein tau (MAPT) whose transcript undergoes complex, regulated alternative splicing, giving rise to several mRNA species. MAPT transcripts are differentially expressed in the nervous system, depending on stage of neuronal maturation and neuron type. MAPT gene mutations have been associated with several neurodegenerative disorders such as Alzheimer's disease, Pick's disease, frontotemporal dementia, cortico-basal degeneration and progressive supranuclear palsy.1) Reynolds, M.R., (2006) Biochemistry 45 (13), 4314-4326. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human MAPT. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| MAPT antibody can be used for detection of MAPT by ELISA at 1:312500. MAPT antibody can be used for detection of MAPT by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MAPT |
|---|---|
| Alternative Names | MAPT DDPAC FLJ31424 FTDP-17 MAPTL MGC138549 MSTD MTBT1 MTBT2 PPND TAU |
| Molecular Weight(MW) | 46 kDa, 40 kDa, 79 kDa, 37 kDa |
Application
Antibody used in WB on N2A at 1:2000 (Lane 1: 10ug N2A expressed human Tau-C Lane 2: 10ug N2A expressed human Tau-E Lane 3: 10ug N2A expressed human Tau-F Lane 4: 10ug N2A expressed human Tau-B Lane 5: 10ug N2A expressed human Fetal-Tau Lane 6: 10ug N2A expressed human Tau-D Lane 7: 10ug N2A expressed human much Fetal-Tau Lane 8: 10ug N2A lysate ).
Application
Antibody used in WB on Human HepG2 at 0.2-1 ug/ml.
Application
Antibody used in IHC on mouse cortex at: 1:1000.| Positive Control | 1) Cat. No. 1211 - HepG2 Cell Lysate |
|---|---|
| Application Notes | MAPT antibody can be used for detection of MAPT by ELISA at 1:312500. MAPT antibody can be used for detection of MAPT by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store MAPT antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
