Application
This antibody stained formalin-fixed paraffin-embedded sections of human normal breast and human breast lobular carcinoma in situ. The recommended concentration is 7.8 ng/mL- 15.625 ng/mL with an overnight incubation at 4˚C. An HRP-labeled polymer detection system was used with a DAB chromogen. Optimal results for these conditions were achieved without antigen retrieval. Optimal concentrationApplication
This antibody stained formalin-fixed paraffin-embedded sections of human normal breast and human breast lobular carcinoma in situ. The recommended concentration is 7.8 ng/mL- 15.625 ng/mL with an overnight incubation at 4˚C. An HRP-labeled polymer detection system was used with a DAB chromogen. Optimal results for these conditions were achieved without antigen retrieval. Optimal concentrationApplication
This antibody stained formalin-fixed paraffin-embedded sections of human normal breast and human breast lobular carcinoma in situ. The recommended concentration is 7.8 ng/mL- 15.625 ng/mL with an overnight incubation at 4˚C. An HRP-labeled polymer detection system was used with a DAB chromogen. Optimal results for these conditions were achieved without antigen retrieval. Optimal concentrationApplication
To detect hMaspin by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMaspin is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.Application
To detect hMaspin by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMaspin is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.Application
To detect hMaspin by sandwich ELISA (using 100ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Human Maspin (38-278) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hMaspin.| Product Name | Maspin Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Maspin was discovered as a mammary tumor suppressor that is expressed in normal mammary epithelium but lost in most breast cancer cell lines. While maspin is related to the serpin family of serine protease inhibitors, it may not function as a protease inhibitor. It plays an essential role in embryonic development through critical roles in cell adhesion. While the precise mechanism of maspin signaling is unclear, the tumor suppressing activity of maspin has been attributed to its ability to inhibit cell invasion/metastasis and angiogenesis, while promoting apoptosis. Nuclear translocation of active IKKα has been shown to repress maspin transcription and promote prostate cancer metastasis. |
| Immunogen | Produced from sera of rabbits immunized with highly pure recombinant Human Maspin. Human Maspin specific antibody was purified by affinity chromatography employing an immobilized Human Maspin matrix. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| ELISA: In a sandwich ELISA (using 100 uL/well antibody solution) a concentration of 0.5 - 2.0 ug/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our biotinylated Anti-Human Maspin as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hMaspin. Western Blot: To detect hMaspin by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMaspin is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | SERPINB5 |
|---|---|
| Alternative Names | PI5 maspin PI5 Serpin B5 Maspin PI-5 |
Application
This antibody stained formalin-fixed paraffin-embedded sections of human normal breast and human breast lobular carcinoma in situ. The recommended concentration is 7.8 ng/mL- 15.625 ng/mL with an overnight incubation at 4˚C. An HRP-labeled polymer detection system was used with a DAB chromogen. Optimal results for these conditions were achieved without antigen retrieval. Optimal concentration
Application
This antibody stained formalin-fixed paraffin-embedded sections of human normal breast and human breast lobular carcinoma in situ. The recommended concentration is 7.8 ng/mL- 15.625 ng/mL with an overnight incubation at 4˚C. An HRP-labeled polymer detection system was used with a DAB chromogen. Optimal results for these conditions were achieved without antigen retrieval. Optimal concentration
Application
This antibody stained formalin-fixed paraffin-embedded sections of human normal breast and human breast lobular carcinoma in situ. The recommended concentration is 7.8 ng/mL- 15.625 ng/mL with an overnight incubation at 4˚C. An HRP-labeled polymer detection system was used with a DAB chromogen. Optimal results for these conditions were achieved without antigen retrieval. Optimal concentration
Application
To detect hMaspin by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMaspin is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Application
To detect hMaspin by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMaspin is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Application
To detect hMaspin by sandwich ELISA (using 100ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Human Maspin (38-278) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hMaspin.| Application Notes | ELISA: In a sandwich ELISA (using 100 uL/well antibody solution) a concentration of 0.5 - 2.0 ug/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our biotinylated Anti-Human Maspin as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hMaspin. Western Blot: To detect hMaspin by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMaspin is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | The lyophilized antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-80˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
