Application
Immunocytochemistry of MATN3 in 3T3 cells with MATN3 antibody at 5 μg/mL.Application
Western blot analysis of MATN3 in 3T3 cell lysate with MATN3 antibody at (A) 1 and (B) 2 μg/mL.| Product Name | MATN3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | MATN3 Antibody: Matrilins (MATNs) are a family of non-collagenous extra-cellular matrix (ECM) proteins consisting of four known members that have been proposed to play key roles in modulating cellular phenotypes during chondrogenesis of mesenchymal stem cells (MSCs). MATN1 and MATN3 are expressed specifically in cartilage and are among the most up-regulated ECM proteins during chondrogenesis. MATN3 is composed of a single N-terminal von Willebrand Factor A (vWFA) domain followed by four epidermal growth factor (EGF) repeats and a coiled-coil domain whereas MATN1 is composed of two vWFA domains separated by one EGF-like domain. MATN1 or MATN3 may play a role in modulating chondrogenesis during the chondrocyte differentiation process. Mutations of this gene have been associated with variety of inherited chondrodysplasias. Recent studies show that aberrant expression and processing of MATN3 are hallmarks of conventional cartilaginous neoplasms.1) Pei M, Luo J, and Chen Q. Enhancing and maintaining matrilins. Osteoarthritis Cartilage2008; 16:1110-7. |
| Immunogen | MATN3 antibody was raised against a 13 amino acid synthetic peptide from near the carboxy terminus of human MATN3. The immunogen is located within amino acids 400 - 450 of MATN3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCWB |
| MATN3 antibody can be used for detection of Matn3 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MATN3 |
|---|---|
| Alternative Names | MATN3 Antibody: HOA OS2 EDM5 DIPOA OADIP Matrilin-3 |
| Sequence Similarities | 2) Cat. No. 17-201 - 3T3/BALB Cell Slide |
Application
Immunocytochemistry of MATN3 in 3T3 cells with MATN3 antibody at 5 μg/mL.
Application
Western blot analysis of MATN3 in 3T3 cell lysate with MATN3 antibody at (A) 1 and (B) 2 μg/mL.| Positive Control | 1) Cat. No. 1282 - 3T3 (NIH) Cell Lysate |
|---|---|
| Application Notes | MATN3 antibody can be used for detection of Matn3 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | MATN3 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | MATN3 Antibody is supplied in PBS containing 0.02% sodium azide. |
