MBP-1 encodes one of three enolase isoenzymes found in mammals; it encodes alpha-enolase, a homodimeric soluble enzyme, and also encodes a shorter monomeric structural lens protein, tau-crystallin. The product of this gene may play a role in the suppression of prostate cancer cell growth by regulating the MEK5-mediated signaling pathway.1) Ghosh, A.K., et al., (1999) Mol. Cell Biol. 19 (4), 2880-6.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human MBP-1.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAWB
MBP-1 antibody can be used for detection of MBP-1 by ELISA at 1:312500. MBP-1 antibody can be used for detection of MBP-1 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Antibody used in WB on Human Jurkat cells at 0.5 ug/ml.
Positive Control
1) Cat. No. 1205 - Jurkat Cell Lysate
Application Notes
MBP-1 antibody can be used for detection of MBP-1 by ELISA at 1:312500. MBP-1 antibody can be used for detection of MBP-1 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store MBP-1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.