Application
Antibody used in WB on Human Jurkat cells at 1.25 ug/ml.| Product Name | MCM4 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | The protein encoded by MCM4 is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 6 and 7 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. The phosphorylation of this protein by CDC2 kinase reduces the DNA helicase activity and chromatin binding of the MCM complex. The MCM4 gene is mapped to a region on the chromosome 8 head-to-head next to the PRKDC/DNA-PK, a DNA-activated protein kinase involved in the repair of DNA double-strand breaks.1) Ishimi, Y., et al., (2003) J. Biol. Chem. 278 (27), 24644-24650. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human MCM4. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| MCM4 antibody can be used for detection of MCM4 by ELISA at 1:12500. MCM4 antibody can be used for detection of MCM4 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. | |
| Species Reactivity | DogHumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | MCM4 |
|---|---|
| Alternative Names | MCM4 NKCD CDC21 CDC54 NKGCD hCdc21 P1-CDC21 |
| Molecular Weight(MW) | 95 kDa, 97 kDa |
Application
Antibody used in WB on Human Jurkat cells at 1.25 ug/ml.| Positive Control | 1) Cat. No. 1205 - Jurkat Cell Lysate |
|---|---|
| Application Notes | MCM4 antibody can be used for detection of MCM4 by ELISA at 1:12500. MCM4 antibody can be used for detection of MCM4 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store MCM4 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt |
