Application
Antibody used in IHC on Human Lung.Application
Antibody used in WB on Human HepG2 at 1.25 ug/ml.| Product Name | MCM6 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | The protein encoded by the MCM6 gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 4 and 7 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. The phosphorylation of the complex by CDC2 kinase reduces the helicase activity, suggesting a role in the regulation of DNA replication.1) Yabuta, N., et al., (2003) Genes Cells 8 (5), 413-421. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human MCM6. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAIHCWB |
| MCM6 antibody can be used for detection of MCM6 by ELISA at 1:62500. MCM6 antibody can be used for detection of MCM6 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. | |
| Species Reactivity | DogHuman |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | MCM6 |
|---|---|
| Alternative Names | MCM6 Mis5 P105MCM MCG40308 |
| Molecular Weight(MW) | 90 kDa |
Application
Antibody used in IHC on Human Lung.
Application
Antibody used in WB on Human HepG2 at 1.25 ug/ml.| Positive Control | 1) Cat. No. 1211 - HepG2 Cell Lysate |
|---|---|
| Application Notes | MCM6 antibody can be used for detection of MCM6 by ELISA at 1:62500. MCM6 antibody can be used for detection of MCM6 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store MCM6 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt |
