Application
Antibody used in WB on Human Daudi cells at 1.25 ug/ml.Application
Antibody used in IHC on Human kidney.| Product Name | MCM7 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | MCM7 encodes a protein that is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 4 and 6 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme.The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 4 and 6 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. Cyclin D1-dependent kinase, CDK4, is found to associate with this protein, and may regulate the binding of this protein with the tumorsuppressor protein RB1/RB. Alternatively spliced transcript variants encoding distinct isoforms have been reported.1) Ren, B., (2006) Oncogene 25 (7), 1090-1098. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human MCM7. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAIHCWB |
| MCM7 antibody can be used for detection of MCM7 by ELISA at 1:312500. MCM7 antibody can be used for detection of MCM7 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | MCM7 |
|---|---|
| Alternative Names | MCM7 MCM2 CDC47 P85MCM P1CDC47 PNAS146 P1.1-MCM3 |
| Molecular Weight(MW) | 81 kDa, 61 kDa |
Application
Antibody used in WB on Human Daudi cells at 1.25 ug/ml.
Application
Antibody used in IHC on Human kidney.| Positive Control | 1) Cat. No. 1224 - Daudi Cell Lysate |
|---|---|
| Application Notes | MCM7 antibody can be used for detection of MCM7 by ELISA at 1:312500. MCM7 antibody can be used for detection of MCM7 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store MCM7 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt |
