Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with MDH1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Flow cytometry analysis of HL-60 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis in HL-60,THP-1 cell line lysates (35ug/lane).| Product Name | MDH1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | MDH1 is localized to the cytoplasm and may play pivotal roles in the malate-aspartate shuttle that operates in the metabolic coordination between cytosol and mitochondria.1) Lee,S.M., et.al., Cell Death Differ. 16 (5), 738-748 (2009) |
| Immunogen | This MDH1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 286-314 amino acids from the C-terminal region of human MDH1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Unpurified |
| Gene Symbol | MDH1 |
|---|---|
| Alternative Names | Malate dehydrogenase cytoplasmic Cytosolic malate dehydrogenase Diiodophenylpyruvate reductase MDH1 MDHA |
| Molecular Weight(MW) | 36 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine |
Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with MDH1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Flow cytometry analysis of HL-60 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis in HL-60,THP-1 cell line lysates (35ug/lane).| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
