Application
Antibody used in WB on Human Jurkat 0.2-1 ug/ml.| Product Name | MDM1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | MDM1 is a nuclear protein similar to the mouse double minute 1 protein. The mouse gene is located in double minute (DM) chromatin particles and is amplified in the mouse transformed 3T3 cell line, and the protein is able to bind to p53. This gene encodes a nuclear protein similar to the mouse double minute 1 protein. The mouse gene is located in double minute (DM) chromatin particles and is amplified in the mouse transformed 3T3 cell line, and the protein is able to bind to p53. In mouse several transcripts have been described for this gene which result from alternative polyadenylation, splicing and exon usage.1) Oh, J.H., (2005) Mamm. Genome 16 (12), 942-954. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human MDM1. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| MDM1 antibody can be used for detection of MDM1 by ELISA at 1:1562500. MDM1 antibody can be used for detection of MDM1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. | |
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MDM1 |
|---|---|
| Alternative Names | MDM1 |
| Molecular Weight(MW) | 25 kDa |
Application
Antibody used in WB on Human Jurkat 0.2-1 ug/ml.| Positive Control | 1) Cat. No. 1205 - Jurkat Cell Lysate |
|---|---|
| Application Notes | MDM1 antibody can be used for detection of MDM1 by ELISA at 1:1562500. MDM1 antibody can be used for detection of MDM1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store MDM1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
