Application
Immunohistochemistry of ME2 in human spleen tissue with ME2 antibody at 5 μg/mL.Application
Immunofluorescence of ME2 in human spleen tissue with ME2 antibody at 20 μg/mL.Application
Western blot analysis of ME2 in human spleen tissue lysate with ME2 antibody at (A) 1 and (B) 2μg/ml.| Product Name | ME2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | ME2 is a homotetrameric, mitochondrial NAD-dependent malic enzyme that catalyzes the oxidative decarboxylation of malate to pyruvate (1). It is related to malic enzyme 1 (ME1), a cytoplasmic NADP-dependent enzyme that generates NADPH for fatty acid biosynthesis (2). The expression of both malic enzymes is reciprocally regulated by p53; this regulation has been shown to modulate metabolism and senescence (3). Certain single-nucleotide polymorphism haplotypes of the ME2 gene have been shown to increase the risk for idiopathic generalized epilepsy (4).1) Loeber G, Infante AA, Maurer-Fogy I, et al. Human NAD(+)-dependent mitochondrial malic enzyme. cDNA cloning, primary structure, and expression in Escherichia coli. J. Biol. Chem. 1991; 266:3016-21. |
| Immunogen | ME2 antibody was raised against a 17 amino acid peptide near the center of human ME2. The immunogen is located within amino acids 340 - 390 of ME2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| ME2 antibody can be used for detection of ME2 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry at 5 μg/mL. For Immunoflorescence start at 20 μg/mL. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ME2 |
|---|---|
| Alternative Names | ME2 Antibody: ODS1 NAD-dependent malic enzyme mitochondrial Malic enzyme 2 NAD-ME |
| Molecular Weight(MW) | Predicted: 64 kDa Observed: 60 kDa |
| Sequence Similarities | 2) Cat. No. 10-901 - Human Spleen Tissue Slide |
| Tissue Specificity | ME2 antibody is human specific. At least two isoforms of ME2 are known to exist; this antibody will detect both isoforms. ME2 antibody is predicted not to cross-react with ME1. |
Application
Immunohistochemistry of ME2 in human spleen tissue with ME2 antibody at 5 μg/mL.
Application
Immunofluorescence of ME2 in human spleen tissue with ME2 antibody at 20 μg/mL.
Application
Western blot analysis of ME2 in human spleen tissue lysate with ME2 antibody at (A) 1 and (B) 2μg/ml.| Positive Control | 1) Cat. No. 1306 - Human Spleen Tissue Lysate |
|---|---|
| Application Notes | ME2 antibody can be used for detection of ME2 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry at 5 μg/mL. For Immunoflorescence start at 20 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | ME2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | ME2 Antibody is supplied in PBS containing 0.02% sodium azide. |
