Application
Western blot analysis in 293 cell line lysates (35ug/lane).| Product Name | MED16 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.1) Sato, S., et al. Mol. Cell 14(5):685-691(2004) |
| Immunogen | This MED16 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 253-281 amino acids from the Central region of human MED16. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MED16 |
|---|---|
| Alternative Names | Mediator of RNA polymerase II transcription subunit 16 Mediator complex subunit 16 Thyroid hormone receptor-associated protein 5 Thyroid hormone receptor-associated protein complex 95 kDa component Trap95 Vitamin D3 receptor-interacting protein comple |
| Molecular Weight(MW) | 97 kDa |
Application
Western blot analysis in 293 cell line lysates (35ug/lane).| Application Notes | For WB starting dilution is: 1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
