Application
Antibody used in WB on Human Brain at 0.2-1 ug/ml.| Product Name | MED21 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | MED21 belongs to the Mediator complex subunit 21 family. It is the component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.1) Andersen, J.S., (2005) Nature 433 (7021), 77-83. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human MED21. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| MED21 antibody can be used for detection of MED21 by ELISA at 1:62500. MED21 antibody can be used for detection of MED21 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. | |
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MED21 |
|---|---|
| Alternative Names | MED21 SRB7 SURB7 hSrb7 |
| Molecular Weight(MW) | 15 kDa |
Application
Antibody used in WB on Human Brain at 0.2-1 ug/ml.| Positive Control | 1) Cat. No. XBL-10123 - Fetal Brain Tissue Lysate |
|---|---|
| Application Notes | MED21 antibody can be used for detection of MED21 by ELISA at 1:62500. MED21 antibody can be used for detection of MED21 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store MED21 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
