Application
Antibody used in WB on Hum. Fetal Muscle at 1 ug/ml.Application
Antibody used in WB on Human 721_B cells at 0.2-1 ug/ml.Application
Antibody used in WB on Hum. Fetal Brain at 1 ug/ml.| Product Name | MED6 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | MED6 is a component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. And mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.1) Pavri, R., (2005) Mol. Cell 18 (1), 83-96. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human MED6. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| MED6 antibody can be used for detection of MED6 by ELISA at 1:312500. MED6 antibody can be used for detection of MED6 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MED6 |
|---|---|
| Alternative Names | MED6 NY-REN-28 ARC33 |
| Molecular Weight(MW) | 28 kDa |
Application
Antibody used in WB on Hum. Fetal Muscle at 1 ug/ml.
Application
Antibody used in WB on Human 721_B cells at 0.2-1 ug/ml.
Application
Antibody used in WB on Hum. Fetal Brain at 1 ug/ml.| Positive Control | 1) 721_B Cell Lysate |
|---|---|
| Application Notes | MED6 antibody can be used for detection of MED6 by ELISA at 1:312500. MED6 antibody can be used for detection of MED6 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store MED6 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
