Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with MEF2C Antibody (S387) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Fluorescent confocal image of Hela cell stained with MEF2C Antibody (S387).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with MEF2C primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated PhaApplication
Western blot analysis in HL-60,K562 cell line lysates (35ug/lane).| Product Name | MEF2C Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | MEF2C is a transcription activator which binds specifically to the MEF2 element present in the regulatory regions of many muscle-specific genes. This protein controls cardiac morphogenesis and myogenesis, and is also involved in vascular development. It may also be involved in neurogenesis and in the development of cortical architecture.1) Konig, S., et al., J. Biol. Chem. 279(27):28187-28196 (2004). Maeda, T., et al., J. Biol. Chem. 277(50):48889-48898 (2002). Maeda, T., et al., Biochem. Biophys. Res. Commun. 294(4):791-797 (2002). Janson, C.G., et al., Brain Res. Mol. Brain Res. 97(1):70-82 (2001). Krainc, D., et al., Genomics 29(3):809-811 (1995). |
| Immunogen | This MEF2C antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 365-394 amino acids from human MEF2C. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IFIHC-PWB |
| For WB starting dilution is: 1:1000 For IF starting dilution is: 1:10~50 For IHC-P starting dilution is: 1:10~50 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MEF2C |
|---|---|
| Alternative Names | Myocyte-specific enhancer factor 2C MEF2C |
| Molecular Weight(MW) | 51 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Pig |
Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with MEF2C Antibody (S387) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Fluorescent confocal image of Hela cell stained with MEF2C Antibody (S387).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with MEF2C primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Pha
Application
Western blot analysis in HL-60,K562 cell line lysates (35ug/lane).| Application Notes | For WB starting dilution is: 1:1000 For IF starting dilution is: 1:10~50 For IHC-P starting dilution is: 1:10~50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
