Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using MEK1 (Ab-221).Application
Western blot analysis of lysed extracts from 293 cells using MEK1 (Ab-221).| Product Name | MEK1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates ERK1 and ERK2 MAP kinases.1) Zebisch A, et al. (2006) Cancer Res; 66 (7): 3401-8. Luciano BS, et al. (2006) J Biol Chem; 279 (50): 52117-23. Wang X, et al. (2003) Oncogene; 22 (1): 109-16. Gopalbhai K, et al. (2003) J Biol Chem; 278 (10): 8118-25. Ling MT, et al. (2002) Oncogene; 21 (55): 8498-505. |
| Immunogen | MEK1 (Ab-221) antibody was raised against a peptide sequence around aa.219~223 (A-N-S-F-V) derived from Human MEK1. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100 | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MAP2K1 |
|---|---|
| Alternative Names | CFC3 MEK1 MKK1 MAPKK1 PRKMK1 JNKK1 MEK4 MKK4 PRKMK4 SEK1 SERK1 SKK1 ERK activator kinase 1 MAP kinase kinase 1 MAP2K1 MAPK/ERK kinase 1 MAPKK 1 |
| Molecular Weight(MW) | 45 kDa |
| Tissue Specificity | This antibody detects endogenous level of total MEK1 protein. |
| Entrez Gene | 5604 |
|---|---|
| Protein Accession | NP_002746.1 |
Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using MEK1 (Ab-221).
Application
Western blot analysis of lysed extracts from 293 cells using MEK1 (Ab-221).| Application Notes | Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
