Application
Western blot analysis of lysed extracts from HepG2 cells untreated or treated with PMA using MEK1 (Phospho-Thr291).| Product Name | MEK1 (phospho Thr291) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates ERK1 and ERK2 MAP kinases.1) Kevin D. Burroughs, et, al. (2003) Mol. Cancer Res ; 1: 312. Michael J. Piatelli, et, al. (2002) J. Biol. Chem ; 277: 12144 - 12150. Margaret M. Morgan, et, al. (2001) J. Immunol ; 167: 5708. Herbert Schramek, et, al. (2003) Am J Physiol Cell Physiol, ; 285: C652 - C661. |
| Immunogen | MEK1 (Phospho-Thr291) antibody was raised against a peptide sequence around phosphorylation site of threonine 291 (P-R-T (p) -P-G derived from Human MEK1. |
| Modification | p-Thr291 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | WB |
| Western Blot: 1:500~1:1000 | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MAP2K1 |
|---|---|
| Alternative Names | CFC3 MEK1 MKK1 MAPKK1 PRKMK1 TYH ERK activator kinase 1 MAP kinase kinase 1 MAP2K1 MAPK/ERK kinase 1 MAPKK 1 |
| Molecular Weight(MW) | 45 kDa |
| Tissue Specificity | This antibody detects endogenous level of MEK1 only when phosphorylated at threonine 291. |
| Entrez Gene | 5604 |
|---|---|
| Protein Accession | NP_002746.1 |
Application
Western blot analysis of lysed extracts from HepG2 cells untreated or treated with PMA using MEK1 (Phospho-Thr291).| Application Notes | Western Blot: 1:500~1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
