Application
Western blot of human T47D cells showing phospho-specific immunolabeling of the ~45k MEK1 protein phosphorylated at Thr386.| Product Name | MEK1 (phospho Thr386) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | MEK 1 (MAP Kinase Kinase, also known as MKK) is an integral component of the MAP kinase cascade that regulates cell growth and differentiation and this pathway also plays a key role in synaptic plasticity in brain. Activated MEK 1 acts as a dual specificity kinase phosphorylating both a threonine and a tyrosine residue on MAP kinase. Conversely there also appears to be a feedback phosphorylation of MEK 1 by MAP kinase. The sites on MEK 1 that are phosphorylated by MAP kinase are Thr292 and Thr386.1) Park, S.H., Zarrinpar, A. and Lim, W.A., "Rewiring MAP kinase pathways using alternative scaffold assembly mechanisms," Science 299 (2003) 1061 - 1064. |
| Immunogen | MEK 1 (Thr386) polyclonal antibody was raised against a synthetic phosphopeptide corresponding to amino acids residues surrounding the phospho Thr386 of human MEK 1. |
| Modification | p-Thr386 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | WB |
| Applications include Dot Blots (DB) and Western Blots (WB). Suitability for Immunohistochemistry (IHC) has not yet been determined. When internally tested under ideal conditions the working dilutions were 1:1000 for DB and WB. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MAP2K1 |
|---|---|
| Alternative Names | CFC3 MEK1 MKK1 MAPKK1 PRKMK1 ERK activator kinase 1 MAP kinase kinase 1 |
| Molecular Weight(MW) | 45 |
| Tissue Specificity | MEK1 antibody is specific for the 45k MEK 1 phosphorylated at Thr386. |
Application
Western blot of human T47D cells showing phospho-specific immunolabeling of the ~45k MEK1 protein phosphorylated at Thr386.| Application Notes | Applications include Dot Blots (DB) and Western Blots (WB). Suitability for Immunohistochemistry (IHC) has not yet been determined. When internally tested under ideal conditions the working dilutions were 1:1000 for DB and WB. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For long term storage –80˚C is recommended, but shorter term storage at –20˚C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol. Stable for one year. |
| Storage Buffer | 100 uL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per mL BSA and 50% glycerol. |
