Application
Western blot analysis of extract from HeLa and JK cells using MEK1/2 Antibody #21428| Product Name | MEK1 / 2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates ERK1 and ERK2 MAP kinases.1) Seger R., Seger D., Lozeman F. J., Ahn N. G., Graves L. M., Campbell J. S., Ericsson L., Harrylock M., Jensen A. M., Krebs E. G. J. Biol. Chem. 267:25628-25631 (1992) Mukherjee S., Keitany G., Li Y., Wang Y., Ball H. L., Goldsmith E. J., Orth K. Science 312:1211-1214 (2006) |
| Immunogen | MEK1/2 antibody was raised against a peptide sequence around aa.235-239 (L-Q-G-T-H) derived from Human MEK1/2. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | WB |
| Western Blot: 1:500~1:1000 | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MAP2K1 |
|---|---|
| Alternative Names | CFC3 MEK1 MKK1 MAPKK1 PRKMK1 |
| Molecular Weight(MW) | 45 kDa |
| Tissue Specificity | This antibody detects endogenous level of total MEK1/2 protein. |
| Entrez Gene | 5604 |
|---|---|
| Protein Accession | NP_002746.1 |
Application
Western blot analysis of extract from HeLa and JK cells using MEK1/2 Antibody #21428| Application Notes | Western Blot: 1:500~1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
