Application
Immunofluorescence of MEK1/2 in mouse brain tissue with MEK1/2 antibody at 20 μg/mL.Application
Immunohistochemistry of MEK1/2 in mouse brain tissue with MEK1/2 antibody at 5 μg/mL.| Product Name | MEK1/2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | The mitogen-activated protein kinase kinase 1 (MAP2K1, also known as MEK1) and the highly homologous MEK2 are members of the dual specificity protein kinase family (1). MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals (2). MEK1 lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, MEK1 is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development (2).1) Zheng CF and Guan KL. Cloning and characterization of two distinct human extracellular signal-regulated kinase activator kinases, MEK1 and MEK2. J. Biol. Chem. 1993; 268:11435-9. |
| Immunogen | MEK1/2 antibody was raised against a 15 amino acid peptide near the center of human MEK1. The immunogen is located within amino acids 240 - 290 of MEK1/2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISA,IF,IHC-P,WB |
| MEK1/2 antibody can be used for detection of MEK1/2 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL. | |
| Species Reactivity | Human,Mouse,Rat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MAP2K1 |
|---|---|
| Alternative Names | Mitogen-activated protein kinase kinase 1 MAP2K1 MAPKK1 MEK1 MKK1 PRKMK1 CFC3 Mitogen-activated protein kinase kinase 2 MAP2K2 MAPKK2 MEK2 MKK2 PRKMK2 CFC |
| Molecular Weight(MW) | Predicted: 43 kDa Observed: 44 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Rabbit: (100%) |
| Tissue Specificity | MEK1/2 antibody is human, mouse, and rat reactive. This antibody detects both MEK1 and MEK2. |
Application
Immunofluorescence of MEK1/2 in mouse brain tissue with MEK1/2 antibody at 20 μg/mL.
Application
Immunohistochemistry of MEK1/2 in mouse brain tissue with MEK1/2 antibody at 5 μg/mL.| Positive Control | 1) Cat. No. 1303 - Human Brain Tissue Lysate |
|---|---|
| Application Notes | MEK1/2 antibody can be used for detection of MEK1/2 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | MEK1/2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | MEK1/2 antibody is supplied in PBS containing 0.02% sodium azide. |
