Application
Confocal immunofluorescent analysis of MEN1 Antibody (T594) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).Application
Western blot analysis of anti-MEN1 Antibody (T594) in Ramos cell line lysates (35ug/lane)Application
Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with MEN1 Antibody (T594) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.| Product Name | MEN1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Menin (MEN1) is a putative tumor suppressor associated with a syndrome known as multiple endocrine neoplasia type 1. In vitro studies have shown menin is localized to the nucleus, possesses two functional nuclear localization signals, and inhibits transcriptional activation by JunD, however, the function of this protein is not known.1) Hashimoto,M., Int. J. Oncol. 33 (2), 333-340 (2008) |
| Immunogen | This MEN1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 584-615 amino acids from human MEN1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IFIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For IF starting dilution is: 1:10~50 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Unpurified |
| Gene Symbol | MEN1 |
|---|---|
| Alternative Names | Menin MEN1 SCG2 |
| Molecular Weight(MW) | 68 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine |
Application
Confocal immunofluorescent analysis of MEN1 Antibody (T594) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
Application
Western blot analysis of anti-MEN1 Antibody (T594) in Ramos cell line lysates (35ug/lane)
Application
Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with MEN1 Antibody (T594) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For IF starting dilution is: 1:10~50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
