Application
Confocal immunofluorescent analysis of Merlin Antibody (N-term)with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).Application
Western blot analysis of Merlin Antibody in NCI-H460 cell line lysates (35ug/lane)Application
Merlin Antibody IHC analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.| Product Name | Merlin Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Merlin is similar to some members of the ERM (ezrin, radixin, moesin) family of proteins that are thought to link cytoskeletal components with proteins in the cell membrane. This protein has been shown to interact with cell-surface proteins, proteins involved in cytoskeletal dynamics and proteins involved in regulating ion transport.1) James,M.F., et.al., Mol. Cell. Biol. 29 (15), 4250-4261 (2009) |
| Immunogen | This Merlin antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 65-95 amino acids from the N-terminal region of human Merlin. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IFIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For IF starting dilution is: 1:10~50 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Unpurified |
| Gene Symbol | NF2 |
|---|---|
| Alternative Names | Merlin Moesin-ezrin-radixin-like protein Neurofibromin-2 Schwannomerlin Schwannomin NF2 SCH |
| Molecular Weight(MW) | 70 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Mouse |
Application
Confocal immunofluorescent analysis of Merlin Antibody (N-term)with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Application
Western blot analysis of Merlin Antibody in NCI-H460 cell line lysates (35ug/lane)
Application
Merlin Antibody IHC analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For IF starting dilution is: 1:10~50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
