Application
Immunocytochemistry of MettL7a in MCF7 cells with MettL7a antibody at 2 μg/mL.Application
Western blot analysis of MettL7A in MCF cell lysate with MettL7A antibody at 2 μg/mL.| Product Name | MettL7A Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | MettL7A Antibody: MettL7A belongs to the methyltransferase superfamily. It is a probable methyltransferase. Methyltransferase is a type of transferase enzyme which transfers a methyl group from a donor to an acceptor. Often methylation occurs on nucleic bases in DNA or amino acids in protein structures. DNA methylation is often utilized to silence and regulate genes without changing the original DNA sequence. DNA methylation may be necessary for normal growth from embryonic stages in mammals. When mutant embryonic stem cells lacking the murine DNA methyltransferase gene were introduced to a germline of mice they caused a recessive lethal phenotype. Methylation may also be linked to cancer development as methylation of tumor suppressor genes promotes tumorgenesis and metastasis.1) Clark HF, Gurney AL, Abaya E, et al. The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment. Genome Res.2003; 13:2265-70. |
| Immunogen | MettL7A antibody was raised against a 13 amino acid synthetic peptide near the center of human MettL7A. The immunogen is located within amino acids 130 - 180 of MettL7A. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIFWB |
| MettL7A antibody can be used for detection of MettL7A by Western blot at 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 2 μg/mL. For immunofluorescence start at 2 μg/mL. | |
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | METTL7A |
|---|---|
| Alternative Names | MettL7A Antibody: AAM-B Methyltransferase-like protein 7A Protein AAM-B |
| Sequence Similarities | 2) Cat. No. 17-012 - MCF7 Cell Slide |
| Tissue Specificity | At least two isoforms of MettL7A are known to exist. This antibody is predicted to not cross-react with MettL7B. |
Application
Immunocytochemistry of MettL7a in MCF7 cells with MettL7a antibody at 2 μg/mL.
Application
Western blot analysis of MettL7A in MCF cell lysate with MettL7A antibody at 2 μg/mL.| Positive Control | 1) Cat. No. 1219 - MCF7 Cell Lysate |
|---|---|
| Application Notes | MettL7A antibody can be used for detection of MettL7A by Western blot at 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 2 μg/mL. For immunofluorescence start at 2 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | MettL7A antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | MettL7A Antibody is supplied in PBS containing 0.02% sodium azide. |
