Application
Immunohistochemistry of MettL7B in human spleen tissue with MettL7B antibody at 2.5 μg/mL.Application
Western blot analysis of MettL7B in human colon tissue lysate with MettL7B antibody at (A) 1 and (B) 2 μg/mL.| Product Name | MettL7B Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | MettL7B Antibody: MettL7B belongs to the methyltransferase superfamily. It is a probable methyltransferase. Methyltransferase is a type of transferase enzyme which transfers a methyl group from a donor to an acceptor. Often methylation occurs on nucleic bases in DNA or amino acids in protein structures. DNA methylation is often utilized to silence and regulate genes without changing the original DNA sequence. DNA methylation may be necessary for normal growth from embryonic stages in mammals. When mutant embryonic stem cells lacking the murine DNA methyltransferase gene were introduced to a germline of mice they caused a recessive lethal phenotype. Methylation may also be linked to cancer development as methylation of tumor suppressor genes promotes tumorgenesis and metastasis.1) Clark HF, Gurney AL, Abaya E, et al. The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment. Genome Res.2003; 13:2265-70. |
| Immunogen | MettL7B antibody was raised against a 13 amino acid synthetic peptide near the amino terminus of human MettL7B. The immunogen is located within amino acids 20 - 70 of MettL7B. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIHC-PWB |
| MettL7b antibody can be used for detection of mettl7b by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. | |
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | METTL7B |
|---|---|
| Alternative Names | MettL7B Antibody: ALDI Methyltransferase-like protein 7B |
| Molecular Weight(MW) | Predicted: 27 kDa Observed: 27 kDa |
| Sequence Similarities | 2) Cat. No. 10-901 - Human Spleen Tissue Slide |
| Tissue Specificity | This MettL7B antibody is predicted to not cross-react with MettL7A. |
Application
Immunohistochemistry of MettL7B in human spleen tissue with MettL7B antibody at 2.5 μg/mL.
Application
Western blot analysis of MettL7B in human colon tissue lysate with MettL7B antibody at (A) 1 and (B) 2 μg/mL.| Positive Control | 1) Cat. No. 1320 – Human Colon Tissue Lysate |
|---|---|
| Application Notes | MettL7b antibody can be used for detection of mettl7b by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | MettL7B antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | MettL7B Antibody is supplied in PBS containing 0.02% sodium azide. |
