MFAP4 is a protein with similarity to a bovine microfibril-associated protein. The protein has binding specificities for both collagen and carbohydrate. It is thought to be an extracellular matrix protein which is involved in cell adhesion or intercellular interactions. The gene encoding MFAP4 is located within the Smith-Magenis syndrome region.This gene encodes a protein with similarity to a bovine microfibril-associated protein. The protein has binding specificities for both collagen and carbohydrate. It is thought to be an extracellular matrix protein which is involved in cell adhesion or intercellular interactions. The gene is located within the Smith-Magenis syndrome region.1) Edelman, E.A., (2007) Clin. Genet. 71 (6), 540-550.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human MFAP4.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAWB
MFAP4 antibody can be used for detection of MFAP4 by ELISA at 1:62500. MFAP4 antibody can be used for detection of MFAP4 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Antibody used in WB on Human MCF-7 at 0.2-1 ug/ml.
Application
Antibody used in IHC on Mouse at 1:500.
Positive Control
1) Cat. No. 1219 - MCF7 Cell Lysate
Application Notes
MFAP4 antibody can be used for detection of MFAP4 by ELISA at 1:62500. MFAP4 antibody can be used for detection of MFAP4 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store MFAP4 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.