Application
Immunocytochemistry of MFN1 in A431 cells with MFN1 antibody at 5 μg/mL.Application
Western blot analysis of MFN1 in A431 cell lysate with MFN1 antibody at 1 μg/ml.| Product Name | MFN1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mitofusin 1 (MFN1) and the related protein MFN2 are mitochondrial membrane GTPase proteins that play a central role in mitochondrial metabolism and may be associated with obesity and/or apoptosis processes (1,2). MFN1 and MFN2 form homotypic and heterotypic complexes and coordinately regulate mitochondrial fusion and are essential for embryonic development (3). When ectopically expressed, MFN1 inhibits the apoptosis-associated amino-terminal conformation change in the apoptotic protein Bax but not its mitochondrial translocation, indicating that MFN1 is involved in the regulating the activation of Bax on the outer mitochondrial membrane (4).1) Chen H, Detmer SA, Ewald AJ, et al. Mitofusins Mfn1 and Mfn2 coordinately regulate mitochondrial fusion and are essential for embryonic development. J. Cell Biol. 2003; 160:189-200. |
| Immunogen | MFN1 antibody was raised against a 17 amino acid peptide near the amino terminus of human MFN1. The immunogen is located within amino acids 20 - 70 of MFN1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCWB |
| WB:1:500~1:2000 ICC:1:100~1:500 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MFN1 |
|---|---|
| Alternative Names | MFN1 Antibody: hfzo1 hfzo2 Mitofusin-1 Fzo homolog |
| Molecular Weight(MW) | Predicted: 82 kDa Observed: 83 kDa |
| Sequence Similarities | 2) Cat. No. 17-002 - A-431 Cell Slide |
| Tissue Specificity | MFN1 antibody is human, mouse and rat reactive. MFN1 antibody is predicted to not cross-react with MFN2. |
Application
Immunocytochemistry of MFN1 in A431 cells with MFN1 antibody at 5 μg/mL.
Application
Western blot analysis of MFN1 in A431 cell lysate with MFN1 antibody at 1 μg/ml.| Positive Control | 1) Cat. No. 1202 - A431 Cell Lysate |
|---|---|
| Application Notes | WB:1:500~1:2000 ICC:1:100~1:500 |
| Form | Liquid |
|---|---|
| Storage Instructions | MFN1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | MFN1 antibody is supplied in PBS containing 0.02% sodium azide. |
