Application
Flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis in MCF-7?HepG2 cell line lysates (35ug/lane).| Product Name | MGAT2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | MGAT2 (UDP-N-acetylglucosamine:alpha-6-D-mannoside-beta-1, 2-N-acetylglucosaminyltransferase II) is a Golgi enzyme catalyzing an essential step in the conversion of oligomannose to complex N-glycans. The enzyme has the typical glycosyltransferase domains: a short N-terminal cytoplasmic domain, a hydrophobic non-cleavable signal-anchor domain, and a C-terminal catalytic domain. Mutations in MGAT2 may lead to carbohydrate-deficient glycoprotein syndrome, type II.1) Yen, C.L., et al., J. Biol. Chem. 278(20):18532-18537 (2003). Chen, S.H., et al., Glycoconj. J. 15(3):301-308 (1998). Tan, J., et al., Am. J. Hum. Genet. 59(4):810-817 (1996). Tan, J., et al., Eur. J. Biochem. 231(2):317-328 (1995). D'Agostaro, G.A., et al., J. Biol. Chem. 270(25):15211-15221 (1995). |
| Immunogen | This MGAT2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 409-439 amino acids from the C-terminal region of human MGAT2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSWB |
| For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MGAT2 |
|---|---|
| Alternative Names | Alpha-1 6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase Beta-1 2-N-acetylglucosaminyltransferase II GlcNAc-T II GNT-II Mannoside acetylglucosaminyltransferase 2 N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase II |
| Molecular Weight(MW) | 52 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Mouse |
Application
Flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis in MCF-7?HepG2 cell line lysates (35ug/lane).| Application Notes | For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
