Application
Immunohistochemistry of MINA in mouse heart tissue with MINA antibody at 5 μg/mL.Application
Western blot analysis of MINA in human heart tissue lysate with MINA antibody at (A) 1 and (B) 2 μg/mL.Application
Immunofluorescence of MINA in mouse heart tissue with MINA antibody at 20 μg/mL.| Product Name | MINA Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | MINA Antibody: MINA is nuclear localized, myc-inducible protein that is thought to play a role in mammalian cell proliferation. Treatment of cancer cells lines such as the colon cancer cell line SW680 with siRNA against MINA inhibits cell growth, demonstrating that MINA may be a potential therapeutic target. MINA regulates several genes related to cell adhesion and metabolism that have also been shown to be regulated by c-Myc, but also regulates other genes whose expression are not modulated by c-Myc such as EGFR, IL-6 and HGF. MINA has also been found to act as a repressor to IL-4 expression in T cells, indicating that it may also play a role in T cell differentiation and genetic variation in T helper type 2 bias.1) Tsuneoka M, Kody Y, Soejima M, et al. A novel myc target gene, mina53, that is involved in cell proliferation. J. Biol. Chem.2002; 277:35450-9. |
| Immunogen | MINA antibody was raised against a 15 amino acid synthetic peptide near the amino terminus of human MINA. The immunogen is located within amino acids 380 - 430 of MINA. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| MINA antibody can be used for detection of MINA by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL. | |
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MINA |
|---|---|
| Alternative Names | MINA Antibody: ROX MDIG NO52 MINA53 Bifunctional lysine-specific demethylase and histidyl-hydroxylase MINA 60S ribosomal protein L27a histidine hydroxylase ROX |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine: (80%) |
Application
Immunohistochemistry of MINA in mouse heart tissue with MINA antibody at 5 μg/mL.
Application
Western blot analysis of MINA in human heart tissue lysate with MINA antibody at (A) 1 and (B) 2 μg/mL.
Application
Immunofluorescence of MINA in mouse heart tissue with MINA antibody at 20 μg/mL.| Positive Control | 1) Cat. No. 1301 - Human Heart Tissue Lysate |
|---|---|
| Application Notes | MINA antibody can be used for detection of MINA by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | MINA antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | MINA Antibody is supplied in PBS containing 0.02% sodium azide. |
Data sheet for OM284741