Application
Top Image: Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue using MKK6 (Phospho-Ser207). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic staining using MKK6 (Phospho-Ser207).Application
Western blot analysis of lysed extracts from HL60 cells untreated or treated with PMA using MKK6 (Phospho-Ser207).| Product Name | MKK6 (phospho Ser207) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | MEK6, MKK6, MAPKK6, PRKMK6, SAPKK3, SAPKK-3, MAP kinase kinase 6, MAP2K6, MAPK/ERK kinase 6, MAPKK 6, MEK6 |
| Product description | Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in MAP kinase p38 exclusively.1) Wang W, et al. (2002) Mol Cell Biol ; 22 (10): 3389-403. Raingeaud J, et al. (1996) Mol Cell Biol; 16 (3): 1247-55. |
| Immunogen | MKK6 (Phospho-Ser207) antibody was raised against a peptide sequence around phosphorylation site of serine 207 (V-D-S (p) -V-A) derived from Human MKK6. |
| Modification | p-Ser207 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IFIHCWB |
| Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200: |
|
| Species Reactivity | HumanRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MAP2K6 |
|---|---|
| Alternative Names | MEK6 MKK6 MAPKK6 PRKMK6 SAPKK3 SAPKK-3 MAP kinase kinase 6 MAP2K6 MAPK/ERK kinase 6 MAPKK 6 MEK6 |
| Molecular Weight(MW) | 41 kDa |
| Tissue Specificity | This antibody detects endogenous level of MKK6 only when phosphorylated at serine207. |
| Entrez Gene | 5608 |
|---|---|
| Protein Accession | NP_002749.2 |
Application
Top Image: Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue using MKK6 (Phospho-Ser207). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic staining using MKK6 (Phospho-Ser207).
Application
Western blot analysis of lysed extracts from HL60 cells untreated or treated with PMA using MKK6 (Phospho-Ser207).| Application Notes | Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
