Application
Western blot analysis of MLF1 using rabbit polyclonal MLF1 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the MLF1 gene.Application
Western blot analysis of MLF1 Antibody in mouse heart tissue lysates (35ug/lane)| Product Name | MLF1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Myeloid leukemia factor 1, Myelodysplasia-myeloid leukemia factor 1, MLF1 |
| Product description | MLF1 is involved in lineage commitment of primary hemopoietic progenitors by restricting erythroid formation and enhancing myeloid formation. The protein interferes with erythopoietin-induced erythroid terminal differentiation by preventing cells from exiting the cell cycle through suppression of CDKN1B/p27Kip1 levels. It suppresses RFWD2/COP1 activity via CSN3 which activates p53 and induces cell cycle arrest. It binds DNA and affects the expression of a number of genes so may function as a transcription factor in the nucleus.1) Li,Z.F., J. Neurol. Sci. 264 (1-2), 77-86 (2008) |
| Immunogen | This MLF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 35-62 amino acids from the N-terminal region of human MLF1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Gene Symbol | MLF1 |
|---|---|
| Alternative Names | Myeloid leukemia factor 1 Myelodysplasia-myeloid leukemia factor 1 MLF1 |
| Molecular Weight(MW) | 31 kDa |
Application
Western blot analysis of MLF1 using rabbit polyclonal MLF1 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the MLF1 gene.
Application
Western blot analysis of MLF1 Antibody in mouse heart tissue lysates (35ug/lane)| Application Notes | For WB starting dilution is: 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
