Application
Western Blot at 1:1000 dilution Lane 1: Jurkat whole cell lysate Lane 2: SW480 whole cell lysate Lysates/proteins at 20 ug per lane.Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.| Product Name | MLK1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Mitogen-activated protein kinase kinase kinase 9, Mixed lineage kinase 1, MAP3K9, MLK1, PRKE1 |
| Product description | MLK1 is a MLK(MAP3K) type protein kinase. The catalytic domain of mixed-lineage kinases (MLKs) kinases have amino acid sequence similarity to both the tyr-specific and the ser/thr-specific kinase classes. In addition to thee unusual nature of the kinase catalytic domains, MLK1 and MLK2 contain 2 leu/ile-zipper motifs and a basic sequence near their C-termini. MLK1 is a member of the neuronal apoptotic JNK/c-Jun pathway acting between Rac1/Cdc42 and MKK4 and -7 in death signaling. MLK1 expression has been documented in human epithelial tumor cell lines of colonic, breast and esophageal origin.1) Durkin, J.T., et al., Biochemistry 43(51):16348-16355 (2004). |
| Immunogen | This MLK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1070-1104 amino acids from the C-terminal region of human MLK1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | MAP3K9 |
|---|---|
| Alternative Names | Mitogen-activated protein kinase kinase kinase 9 Mixed lineage kinase 1 MAP3K9 MLK1 PRKE1 |
| Molecular Weight(MW) | 122 kDa |
Application
Western Blot at 1:1000 dilution Lane 1: Jurkat whole cell lysate Lane 2: SW480 whole cell lysate Lysates/proteins at 20 ug per lane.
Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
