Application
Confocal immunofluorescent analysis of MMP12 Antibody with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).Application
Western blot analysis of anti-hMMP12-R406 Pab pre-incubated without(lane 1) and with(lane 2) blocking peptide in mouse kidney tissue lysates.Application
Western blot analysis of anti-hMMP12-R406 Pab pre-incubated without(lane 1) and with(lane 2) blocking peptide in 293 cell line lysate.Application
Antibody staining MMP12 in human lung tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).Application
Western Blot at 1:2000 dilution + human heart lysate Lysates/proteins at 20 ug per lane.Application
Western Blot at 1:1000 dilution + human lung lysate Lysates/proteins at 20 ug per lane.| Product Name | MMP12 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Macrophage metalloelastase, MME, Macrophage elastase, ME, hME, Matrix metalloproteinase-12, MMP-12, MMP12, HME |
| Product description | Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMPs are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. MMP12 may be involved in tissue injury and remodeling. This protein has significant elastolytic activity. MMP12 can accept large and small amino acids at the P1' site, but has a preference for leucine. Aromatic or hydrophobic residues are preferred at the P1 site, wtih small hydrophobic residues (prefereably alanine) occupying P3. The protein is found in alveolar macrophages but not in peripheral blood monocytes. MMP12 can be induced by exposure to lypopolysaccharide, and is inhibited by dexamethasone.1) Nar, H., et al., J. Mol. Biol. 312(4):743-751 (2001). |
| Immunogen | This MMP12 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 391-420 amino acids from the C-terminal region of human MMP12. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IFIHC-PWB |
| For IHC-P starting dilution is: 1:25 For WB starting dilution is: 1:1000 For IF starting dilution is: 1:10~50: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MMP12 |
|---|---|
| Alternative Names | Macrophage metalloelastase MME Macrophage elastase ME hME Matrix metalloproteinase-12 MMP-12 MMP12 HME |
| Molecular Weight(MW) | 54 kDa |
Application
Confocal immunofluorescent analysis of MMP12 Antibody with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Application
Western blot analysis of anti-hMMP12-R406 Pab pre-incubated without(lane 1) and with(lane 2) blocking peptide in mouse kidney tissue lysates.
Application
Western blot analysis of anti-hMMP12-R406 Pab pre-incubated without(lane 1) and with(lane 2) blocking peptide in 293 cell line lysate.
Application
Antibody staining MMP12 in human lung tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).
Application
Western Blot at 1:2000 dilution + human heart lysate Lysates/proteins at 20 ug per lane.
Application
Western Blot at 1:1000 dilution + human lung lysate Lysates/proteins at 20 ug per lane.| Application Notes | For IHC-P starting dilution is: 1:25 For WB starting dilution is: 1:1000 For IF starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
