Application
Western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the MMP14 antibody detected the MMP14 protein (arrow).Application
Flow cytometric analysis of MCF-7 cells using MMP14 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Antibody staining MMP14 in human lung adenocarcinoma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).Application
Western Blot at 1:2000 dilution Lane 1: A2058 whole cell lysate Lane 2: HT-1080 whole cell lysate Lane 3: mouse lung lysate Lane 4: MCF-7 whole cell lysate Lane 5: NCI-H292 whole cell lysate Lysates/proteins at 20 ug per lane.| Product Name | MMP14 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMPs are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. MMP14 seems to specifically activate progelatinase A, and may thus trigger invasion by tumor cells by activating progelatinase A on the tumor cell surface. Expression is significant in stromal cells of colon, breast, and head and neck.1) Will, H., et al., Eur. J. Biochem. 231(3):602-608 (1995). |
| Immunogen | This MMP14 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 145-174 amino acids from the N-terminal region of human MMP14. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For IHC-P starting dilution is: 1:25 For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50 | |
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MMP14 |
|---|---|
| Alternative Names | Matrix metalloproteinase-14 MMP-14 MMP-X1 Membrane-type matrix metalloproteinase 1 MT-MMP 1 MTMMP1 Membrane-type-1 matrix metalloproteinase MT1-MMP MT1MMP MMP14 |
| Molecular Weight(MW) | 66 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Rat |
Application
Western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the MMP14 antibody detected the MMP14 protein (arrow).
Application
Flow cytometric analysis of MCF-7 cells using MMP14 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Antibody staining MMP14 in human lung adenocarcinoma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).
Application
Western Blot at 1:2000 dilution Lane 1: A2058 whole cell lysate Lane 2: HT-1080 whole cell lysate Lane 3: mouse lung lysate Lane 4: MCF-7 whole cell lysate Lane 5: NCI-H292 whole cell lysate Lysates/proteins at 20 ug per lane.| Application Notes | For IHC-P starting dilution is: 1:25 For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
