Application
Flow cytometric analysis of WiDr cells using MMP15 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Antibody is used in Western blot to detect MMP15 in mouse brain tissue lysate (lane 1) and HL60 cell lysate (lane 2) lysate.Application
Formalin-fixed and paraffin-embedded human colon carcinoma with MMP15 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.| Product Name | MMP15 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Matrix metalloproteinase-15, MMP-15, 3424-, Membrane-type matrix metalloproteinase 2, MT-MMP 2, MTMMP2, Membrane-type-2 matrix metalloproteinase, MT2-MMP, MT2MMP, SMCP-2, MMP15 |
| Product description | Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMPs are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. However, MMP15 is a member of the membrane-type MMP (MT-MMP) subfamily; each member of this subfamily contains a potential transmembrane domain suggesting that these proteins are expressed at the cell surface rather than secreted.1) Jung, M., et al., Prostate 55(2):89-98 (2003). |
| Immunogen | This MMP15 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 165-194 amino acids from the N-terminal region of human MMP15. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Gene Symbol | MMP15 |
|---|---|
| Alternative Names | Matrix metalloproteinase-15 MMP-15 3424- Membrane-type matrix metalloproteinase 2 MT-MMP 2 MTMMP2 Membrane-type-2 matrix metalloproteinase MT2-MMP MT2MMP SMCP-2 MMP15 |
| Molecular Weight(MW) | 76 kDa |
Application
Flow cytometric analysis of WiDr cells using MMP15 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Antibody is used in Western blot to detect MMP15 in mouse brain tissue lysate (lane 1) and HL60 cell lysate (lane 2) lysate.
Application
Formalin-fixed and paraffin-embedded human colon carcinoma with MMP15 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
