MMP-2 is involved in the cleavage of gelatin type I and collagen types IV, V, VII, X.1) Collier, I.E., et al., (1988) J. Biol. Chem. 263:6579-6587..
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human MMP2.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAWB
MMP2 antibody can be used for detection of MMP2 by ELISA at 1:62500. MMP2 antibody can be used for detection of MMP2 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Antibody used in WB on Human at: 1:1000 (Lane 1: 15ug MDA-MB-231 lysate, Lane 2: 15ug MCF7 lysate).
Application Notes
MMP2 antibody can be used for detection of MMP2 by ELISA at 1:62500. MMP2 antibody can be used for detection of MMP2 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store MMP2 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt