Application
Confocal immunofluorescent analysis of MMP7 Antibody with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma; HC = hepatocarcinoma.Application
Western Blot at 1:1000 dilution + HL-60 whole cell lysate Lysates/proteins at 20 ug per lane.| Product Name | MMP7 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Matrilysin, Matrin, Matrix metalloproteinase-7, MMP-7, Pump-1 protease, Uterine metalloproteinase, MMP7, MPSL1, PUMP1 |
| Product description | Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMPs are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. MMP7 degrades proteoglycans, fibronectin, elastin and casein and differs from most MMP family members in that it lacks a conserved C-terminal protein domain. The enzyme is involved in wound healing, and studies in mice suggest that it regulates the activity of defensins in intestinal mucosa. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3.1) Filippov, S., et al., J. Exp. Med. 198(6):925-935 (2003). Rivat, C., et al., FASEB J. 17(12):1721-1723 (2003). Fu, X., et al., J. Biol. Chem. 278(31):28403-28409 (2003). McGuire, J.K., et al., Am. J. Pathol. 162(6):1831-1843 (2003). Sumi, T., et al., Oncol. Rep. 10(2):345-349 (2003). |
| Immunogen | This MMP7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 117-146 amino acids from the Central region of human MMP7. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IFIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For IF starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | MMP7 |
|---|---|
| Alternative Names | Matrilysin Matrin Matrix metalloproteinase-7 MMP-7 Pump-1 protease Uterine metalloproteinase MMP7 MPSL1 PUMP1 |
| Molecular Weight(MW) | 30 kDa |
Application
Confocal immunofluorescent analysis of MMP7 Antibody with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma; HC = hepatocarcinoma.
Application
Western Blot at 1:1000 dilution + HL-60 whole cell lysate Lysates/proteins at 20 ug per lane.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For IF starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
