Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with MOB4A antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Western blot analysis in Daudi,K562 cell line lysates (35ug/lane).Application
Fluorescent image of NIH/3T3 cell stained with MOB4A Antibody .NIH/3T3 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with MOB4A primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (Application
Western blot analysis of lysates from K562, Daudi cell line (from left to right), using MOB4A Antibody at 1:1000 at each lane.| Product Name | MOB4A Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | MOB kinase activator 1B, Mob1 homolog 1A, Mob1A, Mob1B, Mps one binder kinase activator-like 1A, MOB1B, MOB4A, MOBKL1A |
| Product description | MOB4A is similar to the yeast Mob1 protein. Yeast Mob1 binds Mps1p, a protein kinase essential for spindle pole body duplication and mitotic checkpoint regulation. Mob4A regulates NDR1 and NDR2 kinase activity, widely expressed nuclear serine-threonine kinases that have been implicated in cell proliferation and/or tumor progression.1) Bichsel,S.J.,J. Biol. Chem. 279 (34), 35228-35235 (2004) Devroe,E.,J. Biol. Chem. 279 (23), 24444-24451 (2004) |
| Immunogen | This MOB4A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 177-206 amino acids from the C-terminal region of human MOB4A. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IFIHC-PWB |
| For WB starting dilution is: 1:1000 For IF starting dilution is: 1:10~50 For IHC-P starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | MOB1B |
|---|---|
| Alternative Names | MOB kinase activator 1B Mob1 homolog 1A Mob1A Mob1B Mps one binder kinase activator-like 1A MOB1B MOB4A MOBKL1A |
| Molecular Weight(MW) | 25 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Mouse |
Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with MOB4A antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Western blot analysis in Daudi,K562 cell line lysates (35ug/lane).
Application
Fluorescent image of NIH/3T3 cell stained with MOB4A Antibody .NIH/3T3 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with MOB4A primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (
Application
Western blot analysis of lysates from K562, Daudi cell line (from left to right), using MOB4A Antibody at 1:1000 at each lane.| Application Notes | For WB starting dilution is: 1:1000 For IF starting dilution is: 1:10~50 For IHC-P starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
