Application
Immunohistochemistry of MRE11 in rat lung tissue with MRE11 antibody at 5 μg/mL.Application
Western blot analysis of MRE11 in A549 cell lysate with MRE11 antibody at (A) 1 and (B) 2 μg/mL.Application
Immunofluorescence of MRE11 in rat lung tissue with MRE11 antibody at 20 μg/mL.| Product Name | MRE11 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | MRE11 Antibody: ATLD, HNGS1, MRE11, MRE11B, Double-strand break repair protein MRE11A, Meiotic recombination 11 homolog 1, MRE11 homolog 1 |
| Product description | MRE11 Antibody: MRE11 is involved in the repair of DNA double strand breaks as part of a complex that includes the Rad50 and NBS1 protein and is thought to act in the same pathway as the A-T mutated (ATM) protein. By itself, the protein has 3' to 5' exonuclease activity and endonuclease activity. The protein forms a complex with the RAD50 homolog; this complex is required for non-homologous joining of DNA ends and possesses increased single-stranded DNA endonuclease and 3' to 5' exonuclease activities. In conjunction with a DNA ligase, this protein promotes the joining of noncomplementary ends in vitro using short homologies near the ends of the DNA fragments. Mutations in this protein result in a novel ataxia telangiectasia-like disorder (ATLD). Unlike the ATM protein, MRE11 is necessary proper mammalian development.1) Stewart GS, Maser RS, Stankovic T, et al. The DNA double-strand break repair gene hMRE11 is mutated in individuals with an ataxia telangiectasia-like disorder. Cell1999; 99:577-87. |
| Immunogen | MRE11 antibody was raised against a 14 amino acid synthetic peptide from near the amino terminus human MRE11. The immunogen is located within amino acids 40 - 90 of MRE11. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| MRE11 antibody can be used for detection of MRE11 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MRE11A |
|---|---|
| Alternative Names | MRE11 Antibody: ATLD HNGS1 MRE11 MRE11B Double-strand break repair protein MRE11A Meiotic recombination 11 homolog 1 MRE11 homolog 1 |
| Molecular Weight(MW) | Predicted: 78 kDa Observed: 73 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Chicken: (93%) |
Application
Immunohistochemistry of MRE11 in rat lung tissue with MRE11 antibody at 5 μg/mL.
Application
Western blot analysis of MRE11 in A549 cell lysate with MRE11 antibody at (A) 1 and (B) 2 μg/mL.
Application
Immunofluorescence of MRE11 in rat lung tissue with MRE11 antibody at 20 μg/mL.| Positive Control | 1) Cat. No. 1203 - A549 Cell Lysate |
|---|---|
| Application Notes | MRE11 antibody can be used for detection of MRE11 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | MRE11 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | MRE11 Antibody is supplied in PBS containing 0.02% sodium azide. |
