Application
Antibody used in WB on Human 721_B at 2.5 ug/ml.| Product Name | MRPS15 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | MRPS15, DC37, FLJ11564, MPR-S15, RPMS15, S15mt |
| Product description | Mammalian mitochondrial ribosomal proteins help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. MRPS15 is a 28S subunit protein that belongs to the ribosomal protein S15P family. The protein is more than two times the size of its E. coli counterpart, with the 12S rRNA binding sites conserved. Between human and mouse, the protein is the least conserved among small subunit ribosomal proteins.Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that belongs to the ribosomal protein S15P family. The encoded protein is more than two times the size of its E. coli counterpart, with the 12S rRNA binding sites conserved. Between human and mouse, the encoded protein is the least conserved among small subunit ribosomal proteins. Pseudogenes corresponding to this gene are found on chromosomes 15q and 19q.1) Zhang, Z. (2003) Genomics 81 (5), 468-480. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human MRPS15. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| MRPS15 antibody can be used for detection of MRPS15 by ELISA at 1:62500. MRPS15 antibody can be used for detection of MRPS15 by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | MRPS15 |
|---|---|
| Alternative Names | MRPS15 DC37 FLJ11564 MPR-S15 RPMS15 S15mt |
| Molecular Weight(MW) | 30 kDa |
Application
Antibody used in WB on Human 721_B at 2.5 ug/ml.| Positive Control | 1) 721_B Cell Lysate |
|---|---|
| Application Notes | MRPS15 antibody can be used for detection of MRPS15 by ELISA at 1:62500. MRPS15 antibody can be used for detection of MRPS15 by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store MRPS15 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt |
