Application
Immunocytochemistry of MSI2 in EL4 cells with MSI2 antibody at 5 μg/mL.Application
Western blot analysis of MSI2 in EL4 cell lysate with MSI2 antibody at 1 μg/mL.Application
Immunofluorescence of MSI2 in EL4 cells with MSI2 antibody at 20 μg/mL.Application
Immunohistochemistry of MSI2 in mouse brain tissue with MSI2 Antibodyat 5 μg/mL.Application
Immunofluorescence of MSI2 in mouse brain tissue with MSI2 Antibodyat 20 μg/mL.| Product Name | MSI2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | MSI2 Antibody: MSI2H, RNA-binding protein Musashi homolog 2, Musashi-2 |
| Product description | MSI2 Antibody: Musashi2 (MSI2) is an RNA-binding protein that is highly expressed in precursor cells in the ventricular and subventricular zones of the developing mammalian CNS. Like the related MSI1, MSI2 has been suggested to be involved stem cell production and maintenance. MSI2 is the predominant MSI protein in hematopoietic stem cells, and its knockdown leads to reduced engraftment and depletion in vivo. Expression levels of MSI2 are elevated in myeloid leukemia cells lines, and MSI2 appears to cooperate with BCR-ABL1 to induce an aggressive leukemia; the level of MSI2 directly correlates with decreased survival in patients. MSI2 negatively regulates the asymmetric cell fate determinant NUMB, suggesting that this signaling pathway may provide future targets for future therapies.1) Sakakibara S, Nakamura Y, Stoh H, et al. RNA-binding protein Musashi2: developmentally regulated expression in neuronal precursor cells and subpopulations of neurons in mammalian CNS. J. Neurosci. 2001; 21:8091-107 |
| Immunogen | MSI2 antibody was raised against a 15 amino acid synthetic peptide near the amino terminus of human MSI2. The immunogen is located within amino acids 30 - 80 of MSI2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICCIFIHC-P |
| MSI2 antibody can be used for detection of MSI2 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL and immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 5 μg/mL.: |
|
| Species Reactivity | Rat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MSI2 |
|---|---|
| Alternative Names | MSI2 Antibody: MSI2H RNA-binding protein Musashi homolog 2 Musashi-2 |
| Sequence Similarities | 2) Cat. No. 17-207 - EL4 Cell Slide |
| Tissue Specificity | MSI2 antibody is predicted to not cross-react with MSI1. Multiple isoforms of MSI2 are known to exist. |
Application
Immunocytochemistry of MSI2 in EL4 cells with MSI2 antibody at 5 μg/mL.
Application
Western blot analysis of MSI2 in EL4 cell lysate with MSI2 antibody at 1 μg/mL.
Application
Immunofluorescence of MSI2 in EL4 cells with MSI2 antibody at 20 μg/mL.
Application
Immunohistochemistry of MSI2 in mouse brain tissue with MSI2 Antibodyat 5 μg/mL.
Application
Immunofluorescence of MSI2 in mouse brain tissue with MSI2 Antibodyat 20 μg/mL.| Positive Control | 1) Cat. No. 1287 - EL4 Cell Lysate |
|---|---|
| Application Notes | MSI2 antibody can be used for detection of MSI2 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL and immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 5 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | MSI2 antibody can be stored at 4 ˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | MSI2 Antibody is supplied in PBS containing 0.02% sodium azide. |
