Application
Western blot analysis of lysed extracts from HUVEC cells using MSK1 (Ab-376).| Product Name | MSK1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | MSK1, RLPK, MSPK1, RPS6KA5 |
| Product description | Serine/threonine kinase required for the mitogen or stress-induced phosphorylation of the transcription factors CREB (cAMP response element-binding protein) and ATF1 (activating transcription factor-1). Essential role in the control of RELA transcriptional activity in response to TNF. Directly represses transcription via phosphorylation of 'Ser-1' of histone H2A. Phosphorylates 'Ser-10' of histone H3 in response to mitogenics, stress stimuli and epidemal growth-factor (EGF), which results in the transcriptional activation of several immediate early genes, including proto-oncogenes c-fos/FOS and c-jun/JUN. May also phosphorylate 'Ser-28' of histone H3. Mediates the mitogen- and stress-induced phosphorylation of high mobility group protein 14 (HMG-14).1) Roux PP, et al. (2004) Microbiol Mol Biol Rev. Jun; 68 (2): 320-344McCOY C, et al. (2005) Biochem J. Apr 15; 387 (Pt 2): 507-517 |
| Immunogen | MSK1 (Ab-376) antibody was raised against a peptide sequence around aa.374~378 (G-Y-S-F-V) derived from Human MSK1. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | WB |
| Western Blot: 1:500~1:1000: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | RPS6KA5 |
|---|---|
| Alternative Names | MSK1 RLPK MSPK1 RPS6KA5 |
| Molecular Weight(MW) | 90 kDa |
| Tissue Specificity | This antibody detects endogenous level of total MSK1 protein. |
| Entrez Gene | 9252 |
|---|---|
| Protein Accession | NP_004746.2 |
Application
Western blot analysis of lysed extracts from HUVEC cells using MSK1 (Ab-376).| Application Notes | Western Blot: 1:500~1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
