Application
Antibody used in WB on Human MCF-7 at 0.2-1 ug/ml.| Product Name | MSLN Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | MSLN, CAK1, MPF, SMR, SMRP |
| Product description | An antibody that reacts with ovarian cancers and mesotheliomas was used to isolate a cell surface antigen named mesothelin. Although the function of mesothelin is unknown, it may play a role in cellular adhesion and is present on mesothelium, mesotheliomas, and ovarian cancers. This gene encodes a preproprotein that is cleaved into two products, megakaryocyte potentiating factor and mesothelin.An antibody that reacts with ovarian cancers and mesotheliomas was used to isolate a cell surface antigen named mesothelin. Although the function of mesothelin is unknown, it may play a role in cellular adhesion and is present on mesothelium, mesotheliomas, and ovarian cancers. This gene encodes a preproprotein that is cleaved into two products, megakaryocyte potentiating factor and mesothelin. Alternative splicing results in multiple transcript variants.1) Johnson, M.D., (2008) J. Histochem. Cytochem. 56 (6), 579-585. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human MSLN. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| MSLN antibody can be used for detection of MSLN by ELISA at 1:312500. MSLN antibody can be used for detection of MSLN by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MSLN |
|---|---|
| Alternative Names | MSLN CAK1 MPF SMR SMRP |
| Molecular Weight(MW) | 32 kDa |
Application
Antibody used in WB on Human MCF-7 at 0.2-1 ug/ml.| Positive Control | 1) Cat. No. 1219 - MCF7 Cell Lysate |
|---|---|
| Application Notes | MSLN antibody can be used for detection of MSLN by ELISA at 1:312500. MSLN antibody can be used for detection of MSLN by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store MSLN antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
