Application
Antibody used in WB on Human Jurkat cells at 2.5 ug/ml.| Product Name | MTA1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | MTA1 encodes a protein that was identified in a screen for genes expressed in metastatic cells, specifically, mammary adenocarcinoma cell lines. Expression of this gene has been correlated with the metastatic potential of at least two types of carcinomas although it is also expressed in many normal tissues. The role it plays in metastasis is unclear. MTA1 was initially thought to be the 70kD component of a nucleosome remodeling deacetylase complex, NuRD, but it is more likely that this component is a different but very similar protein. These two proteins are so closely related, though, that they share the same types of domains. These domains include two DNA binding domains, a dimerization domain, and a domain commonly found in proteins that methylate DNA. The profile and activity of this gene product suggest that it is involved in regulating transcription and that this may be accomplished by chromatin remodeling.1) Kumar, R., et al., (2002) Nature 418 (6898), 654-657. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human MTA1. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| MTA1 antibody can be used for detection of MTA1 by ELISA at 1:312500. MTA1 antibody can be used for detection of MTA1 by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. | |
| Species Reactivity | DogHumanMouseRatZebra Fish |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | MTA1 |
|---|---|
| Alternative Names | MTA1 |
| Molecular Weight(MW) | 47 kDa, 81 kDa, 59 kDa |
Application
Antibody used in WB on Human Jurkat cells at 2.5 ug/ml.| Positive Control | 1) Cat. No. 1205 - Jurkat Cell Lysate |
|---|---|
| Application Notes | MTA1 antibody can be used for detection of MTA1 by ELISA at 1:312500. MTA1 antibody can be used for detection of MTA1 by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store MTA1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt |
