Application
Western blot analysis in 293 cell line lysates (35ug/lane).Application
Western Blot at 1:2000 dilution Lane 1: MCF-7 whole cell lysate Lane 2: Hela whole cell lysate Lane 3: A431 whole cell lysate Lane 4: mouse brain lysate Lane 5: NIH/3T3 whole cell lysate Lysates/proteins at 20 ug per lane.| Product Name | MTA1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | This gene encodes a protein that was identified in a screen for genes expressed in metastatic cells, specifically, mammary adenocarcinoma cell lines. Expression of this gene has been correlated with the metastatic potential of at least two types of carcinomas although it is also expressed in many normal tissues. The role it plays in metastasis is unclear. It was initially thought to be the 70kD component of a nucleosome remodeling deacetylase complex, NuRD, but it is more likely that this component is a different but very similar protein. These two proteins are so closely related, though, that they share the same types of domains. These domains include two DNA binding domains, a dimerization domain, and a domain commonly found in proteins that methylate DNA. The profile and activity of this gene product suggest that it is involved in regulating transcription and that this may be accomplished by chromatin remodeling. [provided by RefSeq].1) Zhu, X., et al.Thorac Oncol 5(8):1159-1166(2010) |
| Immunogen | This MTA1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 659-687 amino acids from the C-terminal region of human MTA1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000 | |
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MTA1 |
|---|---|
| Alternative Names | Metastasis-associated protein MTA1 MTA1 |
| Molecular Weight(MW) | 81 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Rat |
Application
Western blot analysis in 293 cell line lysates (35ug/lane).
Application
Western Blot at 1:2000 dilution Lane 1: MCF-7 whole cell lysate Lane 2: Hela whole cell lysate Lane 3: A431 whole cell lysate Lane 4: mouse brain lysate Lane 5: NIH/3T3 whole cell lysate Lysates/proteins at 20 ug per lane.| Application Notes | For WB starting dilution is: 1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
